Discover the Cutting Edge Bigfoot Cell Sorter.
Spectral • High Throughput • Biosafety
The Bigfoot Cell Sorter with Sasquatch Software (SQS) provides the power, safety, performance and flexibility for your lab's research today and into the future.Watch the launch video
At a fraction of the size of competitors and a stand-alone footprint with no ancillary requirements, Bigfoot provides unmatched performance in any lab space.
Bigfoot's high-end configurations take advantage of the large number of lasers and detectors to allow spectral unmixing for both analysis and sorting.
High speed sorting is fast and configurable- from 6-way sorting into tubes to 4-way sorting into a 96 well plates or 8-way sorting into 384 well plates, Bigfoot will increase the throughput of your lab.
With a custom designed, integrated Class II biocontainment cabinet, Bigfoot provides safety and protection without compromising high parameter sorter performance.
Bigfoot can be configured with up to nine lasers and 60 detectors providing the versatility for both standard fluorescence detection and spectral unmixing.
SQSoftware combines ease of use with high-end feature performance from setup through experimental design and reporting.
Up To 9 Lasers,
Sorting and Analysis
The first of its kind, Bigfoot allows both spectral sorting and spectral analysis on cell populations.
PowerfulWith custom electronics, firmware, and software designed specifically for high-performance sorting, the Bigfoot has the power and flexibility to tackle your application challenges.
Hot-swappable bulk-fluid bottles allow for a full shift of operation, or for continuous operation by changing during a sort. Optional kits are available for connecting to house DI and waste to reduce the need to fill and empty bulk fluid tanks.
No external support components are needed – goodbye external water baths, vacuum pumps, and compressors.
Optional, adjustable monitor and keyboard arms provide ergonomically-optimized flexibility over multiple heights and positions.
On-board cleaner, decontamination, and sheath concentrate bottles allow for automated sheath mixing as well as system rinsing and cleaning without manual intervention.
Convenient storage is provided for adapters, spare parts, and tubes to help reduce cluttered lab space.
SQSoftware utilizes a clean user interface with intuitive workflow and integrated features allowing simplified acquisition, analysis and sorting.
Consistent path lengths and optimal optical filter layout give you the maximum of optimal detection and sensitivity at every wavelength at each detector.
8-peak bead resolution
Spherotech* 8-peak beads run on the Bigfoot Cell Sorter show signal resolution in key channels.
Analysis of human blood
Data below shows a multi-color panel run on a 9-laser Bigfoot Cell Sorter. The panel shown defines T-cell subsets using lysed whole blood from a normal donor. Antibodies used are CD45 FITC, CD3 Alexa 532, CD4 BUV395, CD8 APC, CD16BV510, CD56 PE, CD19 PE-Cy5.5, CD20 Alexa700, CD25 PE-eFluor610, CD127 BV421, TCRgd PerCP-eFluor710, and CD14 PE-Cy7. The data is shown with spectral unmixing and biexponential scaling.
|Sensitivity||<100 MESF for FITC, PE, APC; <0.2µm FSC resolution with small particle detection module|
|Sample Input||6 tube input positions for 1.5mL, 5mL, or 15mL tubes; temperature control (4-37°C) and agitation for all positions|
|Sort Output||Up to 6-way sorting into tubes; configurable tube holders include 1.5mL, 5mL, 15mL, and 50mL adapters. Multi-way micro-well plate sorting up to 1536 wells. Temperature control (4-37°C) for all media types.|
|Nozzle||50, 70, 100, 120, and 150µm ceramic nozzle tips with adjustable pressure settings|
|Biocontainment||Integrated Class II biocontainment cabinet for protection of sample and user. Separate AES for sort chamber evacuation.|
|Excitation||Up to nine lasers (all 100mW). Options include:
|Detection||Up to 60 parameters including FSC and SSC|
|Speed||100,000 eps acquisition and 70,000 eps sorting with all 60 parameters|
Simultaneous measured peak, area, and width for every channel
24 Bit data for peak and area to maximize dynamic range
True measured width at half-height
Low noise converters and proprietary digital processing reduce channel noise
|Bulk fluids||2 x 4L bulk fluid tanks on board for DI water and waste. 4L bottle for sheath or sheath concentrate. Two 1L on board cleaners. Optional kits available for connecting to house DI and waste.|
|Power||100-240VAC 50/60Hz; 800W max|
|Dimensions||99cm W x 99cm D x 177cm H|
|Operating system||Windows* 10|
|FCS format||FCS 3.1|
|QC||Automated quality control and drop delay with multi-peak, on-board calibration beads|
|Workstation||8-core i7-9700k processor, 32GB RAM, and 2TB PCIe SSD|